Reporter

Part:BBa_K223047:Design

Designed by: Suzanne Bartram   Group: iGEM09_Stanford   (2009-10-17)


SoxR - SoxS - GFP Reporter with Lac Promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 880
    Illegal suffix found in sequence at 714
    Illegal SpeI site found at 965
    Illegal PstI site found at 979
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 229
    Illegal EcoRI site found at 880
    Illegal SpeI site found at 715
    Illegal SpeI site found at 965
    Illegal PstI site found at 729
    Illegal PstI site found at 979
    Illegal NotI site found at 235
    Illegal NotI site found at 722
    Illegal NotI site found at 886
    Illegal NotI site found at 972
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 229
    Illegal EcoRI site found at 880
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 229
    Illegal suffix found in sequence at 715
    Illegal EcoRI site found at 880
    Illegal XbaI site found at 895
    Illegal SpeI site found at 965
    Illegal PstI site found at 979
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 229
    Illegal EcoRI site found at 880
    Illegal XbaI site found at 244
    Illegal XbaI site found at 895
    Illegal SpeI site found at 715
    Illegal SpeI site found at 965
    Illegal PstI site found at 729
    Illegal PstI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1654


Design Notes

Tested on both a high (pSB1A2) and low (pSB4A5) copy plasmid. Both high and low copy vector parts to be submitted to the registry.


Source

Stanford iGEM 2009

References